Figure_3.tif (2.16 MB)

Light-elicited inhibition of hilar GABAergic interneurons significantly increased cFos-positive neurons in the dentate gyrus.

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posted on 2013-02-20, 03:02 authored by Yaisa Andrews-Zwilling, Anna K. Gillespie, Alexxai V. Kravitz, Alexandra B. Nelson, Nino Devidze, Iris Lo, Seo Yeon Yoon, Nga Bien-Ly, Karen Ring, Daniel Zwilling, Gregory B. Potter, John L. R. Rubenstein, Anatol C. Kreitzer, Yadong Huang

(A, B) Confocal images of neurons positive for cFos (red) and eNpHR3.0-eYFP (green) on the side of DG without (A) or with (B) laser illumination in I12b-Cre mice injected with AAV1-DIO-eNpHR3.0-eYFP virus. (C) Quantification of cFos-positive dentate granule neurons on the side of DG without or with laser illumination in I12b-Cre mice injected with AAV1-DIO-eNpHR3.0-eYFP virus. Values are mean ± SEM (n = 6, *p<0.05, two tailed and paired t-test). (D, E, G, H) Confocal images of CA1 (D, E) and CA3 (G, H) neurons positive for cFos (red) and eNpHR3.0-eYFP (green) on the side of the hippocampus without (D, G) or with (E, H) laser illumination in the hilus of the dentate gyrus in I12b-Cre mice injected with AAV1-DIO-eNpHR3.0-eYFP virus. (F, I) Quantification of cFos-positive CA1 (F) and CA3 (I) neurons on the side of the hippocampus without or with laser illumination in the hilus of the dentate gyrus in I12b-Cre mice injected with AAV1-DIO-eNpHR3.0-eYFP virus. Values are mean ± SEM (n = 6 mice).